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Zeitschriftenbeiträge (peer-reviewed)

2020

Maas, P.; Grzegrzółka, B.; Kreß, P.; Oberle, M.; Judas, M.; Kremer-Rücker, P. (2020): Prediction of body composition in mirror carp (Cyprinus carpio) by using linear measurements in vivo and computed tomography post-mortem. Archives Animal Breeding 2020 (63), S.69-80.
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2019

Maas, P.; Grzegrzółka, B.; Kreß, P.; oberle, m.; Kremer-Rücker, P. (2019): In vivo – determination of the fat content in mirror carps (Cyprinus carpio) using ultrasound, microwave and linear measurements. Aquaculture 2019 (512), S.734359.
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Carps are the third largest species in aquaculture worldwide and belong to the fish family ‘cyprinids’, which make up the largest part of aquaculture production. The production of lean carp is one of the main goals in the carp farming business. Carps are usually traded alive; in order to ensure product quality and achieve a high consumer acceptance, it is important to estimate the fat content of the living fish. Therefore, during this study, a total of 250 living carps were examined using a mobile ultrasound device. Additionally, a microwave technology based Fish Fatmeter (Distell, Fauldhouse, Scotland, UK) was used to determine the fat content of the living fish. Further measurements included weight, lengths, height and circumferences. The ultrasound measurements were performed on non-sedated fish using narrow water-filled containers. Two sagittal images per fish were taken to measure the backfat thickness at defined locations. Subsequently, the fish were taken out of the water in order to determine the fat content using the Fish Fatmeter. Weight and linear measurements were taken. After the in vivo measurements, about 10 fish per pond, in total 51 fish, were slaughtered and the fillets were analysed chemically. Linear regression models were developed. The Fish Fatmeter turned out to be an accurate method to determine the fat content of the fillet in mirror carps (R2 = 0.95). Regarding the ultrasound measurement of the backfat thickness, moderate correlations between ultrasound and Fish Fatmeter were achieved (R2 = 0.33–0.45). Pearson's correlation for linear measurement and the Fish Fatmeter showed negative prediction. In order to evaluate the relative backfat thickness, the ultrasound measurement was divided by linear measurements. The best correlation was found using the ultrasound measurement point where the backfat thickness reached a constant thickness divided by the circumference around the thinnest part of the tail fin (R2 = 0.74). In the next step, the carps were divided into groups of ten fish per pond. The mean Fish Fatmeter measurement and ultrasound measurement divided by circumference was calculated and correlated achieving an R2 of 0.92. Based on these findings, it seems plausible to estimate the fat content using a small sample of ten fish with an ultrasound device and a measuring tape.

Bertsch, T.; Lüdecke, J.; Nausch, L.; Landsteiner, K. (2019): The Discovery of the ABO Blood Group System and its Value for Teaching Medical Students. Clin Lab. 2019 Jun 1;65 (6).
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With his discovery of the ABO blood group system, Karl Landsteiner laid the foundation for modern day transfusion medicine. This discovery represents the basic knowledge for every blood transfusion. In recent years, certain blood groups have been linked to an increased risk for certain diseases. Hence, teaching the blood group serology is a relevant issue in medical education. In this review, we report about the history of the discovery of the blood groups by Landsteiner, the link of different blood groups to certain diseases and our experiences regarding teaching to medical students.

Wilbois, K.; Schmidt, J. (2019): Reframing the Debate Surrounding the Yield Gap between Organic and Conventional Farming. Agronomy 9 (2), S.82.
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In this article, we review the literature regarding the yield gap between organic and conventional agriculture and then reflect on the corresponding debate on whether or not organic farming can feed the world. We analyze the current framework and highlight the need to reframe the yield gap debate away from “Can organic feed the world?” towards the more pragmatic question, “How can organic agriculture contribute to feeding the world?”. Furthermore, we challenge the benchmarks that are used in present yield comparison studies, as they are based on fundamentally distinct paradigms of the respective farming methods, and then come up with a novel model to better understand the nature of yield gaps and the benchmarks that they are premised on. We thus conclude that, by establishing appropriate benchmarks, re-prioritizing research needs, and focusing on transforming natural resources rather than inputs, organic systems can raise their yields and play an ever-greater role in global sustainable agriculture and food production in the future.

2018

Hunaefi, D.; Smetanska, I.; Juliana, N.; Gruda, N. (2018): Effect of ultraviolet and ultrasonic on potential antidiabetic activity of in vitro shoot cultures of Orthosiphon aristatus. IOP Conference Series Earth and Environmental Science 2018 (207 (1)), S.012008.
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Moon, T.; Sheehe, J.; Nukareddy, P.; Nausch, L.; Wohlfahrt, J.; Matthews, D.; Blumenthal, D.; Dostmann, W. (2018): An N-terminally truncated form of cyclic GMP-dependent protein kinase Iα (PKG Iα) is monomeric, autoinhibited, and provides a model for activation. J Biol Chem. 2018 Mar 30.

The type I cGMP-dependent protein kinases (PKG I) serve essential physiological functions, including smooth muscle relaxation, cardiac remodeling, and platelet aggregation. These enzymes form homodimers through their N-terminal dimerization domains, a feature implicated in regulating their cooperative activation. Previous investigations into the activation mechanisms of PKG I isoforms have been largely influenced by structures of the cAMP-dependent protein kinase (PKA). Here, we examined PKG Iα activation by cGMP and cAMP by engineering a monomeric form that lacks N-terminal residues 1-53 (Δ53). We found that the construct exists as a monomer as assessed by whole-protein MS, size-exclusion chromatography, and small-angle X-ray scattering (SAXS). Reconstruction of the SAXS 3D envelope indicates that Δ53 has a similar shape to the heterodimeric RIα-C complex of PKA. Moreover, we found that the Δ53 construct is autoinhibited in its cGMP-free state and can bind to and be activated by cGMP in a manner similar to full-length PKG Iα as assessed by surface plasmon resonance (SPR) spectroscopy. However, we found that the Δ53 variant does not exhibit cooperative activation, and its cyclic nucleotide selectivity is diminished. These findings support a model in which, despite structural similarities, PKG Iα activation is distinct from that of PKA, and its cooperativity is driven by in trans interactions between protomers.

2017

Horneburg, B.; Seiffert, S.; Schmidt, J.; Messmer, M.; Wilbois, K. (2017): Weed tolerance in soybean: a direct selection system. Plant Breeding 136, S.372-378.
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Weed competition can severely reduce soybean (Glycine max (L.) Merr.) yields, particularly in organic systems. An efficient screening and breeding approach is needed to increase breeding progress for weed tolerance. This study sought to (i) establish a system for direct selection of competitive genotypes, (ii) evaluate genotypic differences in weed tolerance among six early‐maturing genotypes and (iii) assess the contribution of selected morphological traits to weed tolerance. A direct selection system providing two different levels of weed competition through all development stages of a soybean crop was developed, using mixtures of selected crop species as sown competitors. Two resulting mixtures induced intermediate (<30%) and strong (>50%) yield reduction, respectively. This selection system can be applied in screening and breeding programmes to facilitate breeding for weed tolerance. No significant difference in weed tolerance was detected between six soybean genotypes of maturity groups 000 to 00. Morphological traits that might influence competitive ability, for example light absorption, leaf area and lateral shoots, were assessed, and their potential for indirect selection for weed tolerance is discussed.

Gabr, A.; Mabrock , H.; Smetanska, I.; Emam , A.; Mohamed , E. (2017): Determination of lignans, phenolic acids and antioxidant capacity in transformed hairy root culture of Linum usitatissimum. Natural Product Research, Taylor and Francis, New York.
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Smetanska, I. (2017): Bunt, gesund, vielfältig … Der aufwendige Trend zu Fresh-Cut-Salaten.. Journal Culinarie 2017 (25), S.58-64.

Bernau, M.; Kremer-Rücker, P.; Kreuzer, L.; Schwanitz, S.; Cussler, K.; Hoffmann, A.; Scholz, A. (2017): Magnetic resonance imaging to detect local tissue reactions after vaccination in sheep in vivo. Veterinary Record Open 4.
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2016

Zimmer, S.; Messmer, M.; Haase, T.; Piepho, H.; Mindermann, A.; Schulz, H.; Habekuß, A.; Ordon, F.; Wilbois, K.; Heß, J. (2016): Effects of soybean variety and Bradyrhizobium strains on yield, protein content and biological nitrogen fixation under cool growing conditions in Germany. European Journal of Agronomy 72, S.38-46.
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Soybean (Glycine max (L.) Merr.) is able to fix atmospheric nitrogen in symbiosis with the bacteria Bradyrhizobium japonicum. Because these bacteria are not native in European soils, soybean seeds must be inoculated with Bradyrhizobium strains before sowing to fix nitrogen and meet their yield potential. In Central Europe soybean cultivation is still quite new and breeding of early maturing soybean varieties adapted to cool growing conditions has just started.

Smetanska, I.; Blaut, M.; Mabrock, H. (2016): Lignan accumulation in callus and Agrobacterium rhizogenes-mediated hairy root cultures of flax (Linum usitatissimum). Cell, Tissue and Organ Culture: Journal of Plant Biotechnology 2016, S.1-13.
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Smetanska, I.; Helfert, J.; Shevchenko, Y.; Mohdaly , A. (2016): Antioxidant activity of apple peels. . Mechanic and Technology. (1), S.27-36.

Sytar , O.; Shevchenko, Y.; Smetanska, I.; Wendt, A. (2016): Antoxidant activity and phenolics composition in stevia rebaudiana plants of different orgin. . Journal of Microbiology, Biotechnology and Food Sciences .
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Smetanska, I.; Kilian, M.; Groß, É.; Stankowski, S. (2016): Product design in the development of recipes of gluten-free and sugar- reduced bakery products. Mechanic and Technology (1), S.23-27 .

Smetanska, I. (2016): Enhancement of silymarin and phenolic compounds accumulation in tissue culture of Milk thistle using elicitors feeding and hairy root cultures. . Genetic Engineering and Biotechnology Journal 2016 (14(2) ).
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Jaquemoth, M.; Greiner, M.; Hufnagel, R.; Frölich, N. (2016): Gemeinschaftsverpflegung in Bayern – repräsentative Befragung zu Kenndaten von GV-Einrichtungen. Hauswirtschaft und Wissenschaft (3), S.124 ff.

2015

Gronle, A.; Lux, G.; Böhm, H.; Schmidtke, K.; Wild, M.; Demmel, M.; Brandhuber, R.; Wilbois, K.; Hess, J. (2015): Effect of ploughing depth and mechanical soil loading on soil physical properties, weed infestation, yield performance and grain quality in sole and intercrops of pea and oat in organic farming. Soil Tillage Res 148, S.59-73.
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The effect of ploughing depth and mechanical soil loading on the performance of pea sole crops, oat sole crops and pea–oat intercrops was investigated in field experiments under organic farming conditions at two sites in Germany in 2009 and 2010. The two ploughing depths were short-term shallow ploughing to a soil depth of 7–10 cm and deep ploughing to 25–30 cm. Wheel loads of 26 and 45 kN, which correspond to typical rear wheel loads of field machinery used during sowing operations, were compared to an uncompacted control. Shallow ploughing resulted in a greater penetration resistance in the 14–28 cm soil layer compared to deep ploughing. An increase in mechanical soil loading intensity increased the bulk density and decreased the air capacity in the 10–15 cm soil layer, whereas the penetration resistance was not affected. The annual weed infestation in pea sole crops was higher after shallow than after deep ploughing at both sites. Pea–oat intercrops compensated for the higher weed infestation after shallow ploughing at one site due to their excellent weed suppressive ability. Dependent on oat productivity, pea–oat intercrops produced comparable or higher grain and protein yields than pea sole crops. Intercropped pea yield components and grain protein yields were significantly lower than those of sole cropped peas. The ploughing depth did not affect pea grain yields in either year and oat yields in 2009. Due to a better emergence, the grain and protein yield of sole and intercropped oats were significantly higher after shallow ploughing in 2010. Mechanical soil loading did not have any effect on the yield performance of pea sole crops, oat sole crops and pea–oat intercrops in 2009. In 2010, mechanical soil loading of 26 kN and 45 kN decreased the pea grain yield by 12.1% and 20.8% respectively, regardless of sole or intercropped. Neither the grain yield nor the grain quality of sole and intercropped oats was affected by the mechanical soil loading in 2010. Grain and crude protein yields of total crop stands decreased with increasing mechanical soil loading after deep ploughing, whereas no significant differences were revealed after shallow ploughing. The present study confirms the positive qualities of pea–oat intercrops with regard to weed suppression and plant performance. Shallow ploughing mitigates the risk of a decrease in crop performance caused by heavy field traffic and provides an alternative to deep ploughing even in low weed competitive organically farmed grain legumes.

Kastell , A.; Zrenner , R.; Schreiner , M.; Kroh , L.; Ulrichs, C.; Smetanska, I.; Mewis, I. (2015): Metabolic engineering of aliphatic glucosinolates in hairy root cultures of Arabidopsis thaliana. . Plant Molecular Biology Reporter 33 (3), 598-608 2015 (33).
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Mohdali , A.; Smetanska, I.; Mohamed, A. (2015): Chemical composition, physicochemical properties and fatty acid profile of Tiger Nut (Cyperus esculentus L) seed oil as affected by different preparation methods . International Food Research Journal 22 (5), S.1931-1938.

Mohdali , A.; Mahmoud , A.; Smetanska, I.; Roby , M.; Ramadan , M. (2015): Phenolic extract from propolis and bee pollen: composition, antioxidant and antibacterial activies.5 . Journal of Food Biochemistry.
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Smetanska, I.; Shevchenko, Y.; Kilian , M.; Groß, E.; Voytsekhivskiy , V. (2015): Experimental design and development of recipes for obtaining of fructose-free jams. . Scientific Reports of National University of Natural Bio-ressources, UDC 664.165 2015 (8), S.1-11.

Smetanska, I.; Sytar , O. (2015): The contribution of buckwheat genetic resources to health and dietary diversity. , , 5:2. Journal of Biotechnology & Biomaterials 2015 (5), S.2.
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Ade-Omowaye , B.; Smetanska, I.; Tucker , G. (2015): Nutritional potential of nine underexploited legumes in Southwest Nigeria. . International Food Research Journal 22 (2), S.798-806.

Nermeen , M.; Smetanska, I. (2015): Study the effect of hairy root transformation on rapid growth (growth morphology) of Nepeta cataria in vitro cultures. Journal of Innovations in Pharmaceuticals and Biological Sciences, S.440-450.

Schmidt, J.; Messmer, M.; Wilbois, K. (2015): Beneficial microorganisms for soybean (Glycine max (L.) Merr), with a focus on low root-zone temperatures. Plant and Soil 397, S.411-445.
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Background

Heightened interest in biologically-based methods of raising crop yields has driven research into beneficial microorganisms that can be used to improve crop growth and productivity.

Scope

This review addresses the potential of rhizobia, vesicular-arbuscular mycorrhizae (VAM), and plant-growth-promoting rhizobacteria to improve growth of soybean (Glycine max (L.) Merr) under temperate conditions. Mechanisms of action of beneficial microorganisms and considerations for utilization at all root-zone temperatures (RZTs) are reviewed. Subsequent sections address current knowledge on the inhibition of soybean growth at low RZTs and the potential of beneficial microorganisms to alleviate low temperature stress.

Conclusions

The three categories of beneficial microorganisms discussed differ in their modes of action and have shown potential for additive or synergistic growth promotion of soybean at all RZTs. At low RZT, pot and field studies have identified strains of rhizobia and PGPR, as well as certain phytohormones, that ameliorate the inhibitory effects of cold stress on soybean growth through a variety of mechanisms. Wider use of these treatments could aid the expansion of soybean cultivation in cold climates.


2014

Fuchs, J.; Thürig, B.; Brandhuber, R.; Bruns, C.; Finckh, M.; Fließbach, A.; Mäder, P.; Schmidt, H.; Vogt-Kaute, W.; Wilbois, K.; Tamm, L. (2014): Evaluation of the causes of legume yield depression syndrome using an improved diagnostic tool. Applied Soil Ecology 79, S.26-36.
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The aim of the study was to establish a diagnostic tool to narrow down the causes for pea yield depressions. A differential two-level diagnostic test system was established under controlled conditions using peas (Pisum sativum L.) as test plants. Soils from 22 organically managed sites with unexplained moderate to high pea yield losses were tested in level 1 diagnostics (γ-irradiation to eliminate potentially harmful organisms, nutrient additions to compensate for potential nutrient deficiencies or activated charcoal amendment to bind and thereby to immobilize potentially phytotoxic compounds). Results showed that organisms harmful to the test plant were the primary cause of limited germination and growth in most of the sampled soils, whereas a positive effect of nutrient addition was rarely found and toxins were not involved. Level 2 diagnostics (pesticides targeting ascomycetes, oomycetes, Rhizoctonia spp., nematodes) further narrowed down the organisms involved in yield depressions. Oomycetes were identified as the primary reason for limited germination rates, and, in some soils, also for limited growth of established seedlings. In other soils, a multitude rather than a single group of pathogens was involved in limited growth. Plant-pathogenic nematodes were never found to be limiting for crop growth parameters. Harmful effects of pesticides were found in several soils, hinting at an important role of beneficial soil organisms in the suppression of pathogens causing yield depression in legumes. The bioassay used in the present study was robust and could thus serve as a low-cost tool for agricultural advisors and farmers to predict the risk of yield losses in legumes and to narrow down causes, helping them to develop appropriate strategies.

Kastell , A.; Smetanska, I.; Cai, Z. (2014): Chitosan or yeast extract enhance the accumulation of eight phenolic acids in cell suspension cultures of Malus × domestica Borkh.. The Journal of Horticultural Science and Biotechnology 89 (93-99).

Sytar , O.; Smetanska, I.; Borankulova, A.; Hemmerich, I.; Rauh, C. (2014): Effect of chlorocholine chlorid on phenolic acids accumulation and polyphenols formation of buckwheat plants. Biological research 47 (19).
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2013

Kastell , A.; Ulrichs , C.; Cai, Z.; Mewis, I.; Smetanska, I. (2013): Effects of phytohormones and jasmonic acid on glucosinolate content in hairy root cultures of Sinapis alba and Brassica rapa . Applied Biochemistry and Biotechnology 169, S.624- 635 .

Hunaefi, D.; Akumo, D.; Gruda, N.; Smetanska, I. (2013): The effect of lactic acid fermentation on rosmarinic acid and antioxidant properties of in vitro shoot culture of Orthosiphon aristatus as a model study. . Food Biotechnology (27), S.152–177.
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Smetanska, I.; Shevchenko, Y.; Demina , N.; Kanarskyj, A. (2013): Study of anti-oxidative activity of stevia extracts. . Proceedings of the Kazan Technological University. . Vol 20 : 144-147 (20), S.144-147.

Hunaefi, D.; Riedel , H.; Gruda, N.; Akumo, D.; Smetanska, I. (2013): Improvement in antioxidant activities by lactic acid bacteria. . Food Biotechnology (4), S.279-302.
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Cai, Z.; Kastell , A.; Speiser , C.; Smetanska, I. (2013): Enhanced Resveratrol Production in Vitis vinifera Cell Suspension Cultures by Heavy Metals without Loss of Cell Viability. . Applied Biochemistry and Biotechnology .
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Ravichandran, K.; Saw , N.; Mohdaly, A.; Kastell , A.; Riedel , H.; Cai, Z.; Knorr, D.; Smetanska, I. (2013): Impact of processing of red beet on betalain content and antioxidant activity. . Food Research International (50), S.670-675.
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Kastell , A.; Smetanska, I.; Schreiner , M.; Mewis, I. (2013): In vitro cultures and plants of Arabidopsis thaliana exhibit distinct glucosinolate and gene expression profiles. . Plant Cell, Tissue and Organ Culture (PCTOC): Journal of Plant Biotechnology, 115, S.45-54.
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Yang, Q.; Nausch, L.; Martin, G.; Keller, G.; Doublié, S. (2013): Crystal Structure of Human Poly(A) Polymerase Gamma Reveals a Conserved Catalytic Core for Canonical Poly(A) Polymerases. J Mol Biol. 2013 Sep 25.

In eukaryotes, the poly(A) tail added at the 3′ end of a mRNA precursor is essential for the regulation of mRNA stability and the initiation of translation. Poly(A) polymerase (PAP) is the enzyme that catalyzes the poly(A) addition reaction. Multiple isoforms of PAP have been identified in vertebrates, which originate from gene duplication, alternative splicing, or post-translational modifications. The complexity of PAP isoforms suggests that they might play different roles in the cell. Phylogenetic studies indicate that vertebrate PAPs are grouped into three clades termed α, β and γ, which originated from two gene duplication events. To date, all the available PAP structures are from the PAPα clade. Here, we present the crystal structure of the first representative of the PAPγ clade, human PAPγ, bound to cordycepin triphosphate (3′dATP) and Ca2+. The structure revealed that PAPγ closely resembles its PAPα ortholog. An analysis of residue conservation reveals a conserved catalytic binding pocket, whereas residues at the surface of the polymerase are more divergent.

2012

Eckhardt, K. (2012): Technical Note: Analytical sensitivity analysis of a two parameter recursive digital baseflow separation filter. Hydrology and Earth System Sciences (HESS) 16, S.451-455.
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A sensitivity analysis for a well-established baseflow separation technique, a two parameter recursive digital filter, is presented. The sensitivity of the calculated baseflow index to errors or uncertainties of the two filter parameters and of the initial baseflow value is analytically ascertained. It is found that the influence of the initial baseflow value is negligible for long time series. The propagation of errors or uncertainties of the two filter parameters into the baseflow index is expressed by a dimensionless sensitivity index, the ratio between the relative error of the baseflow index and the relative error of the respective parameter. Representative index values are derived by application of the resulting equations to 65 North American catchments. In the mean the parameter a, the recession constant, has a stronger influence on the calculated baseflow index than the second filter parameter BFImax. This is favourable in that a can be determined by a recession analysis and therefore should be less uncertain. Whether this finding also applies for a specific catchment can easily be checked by means of the derived equations.

Nausch, L.; Bonev, A.; Heppner, T.; Tallini, Y.; Kotlikoff, M.; Nelson, M. (2012): Sympathetic nerve stimulation induces local endothelial Ca2+ signals to oppose vasoconstriction of mouse mesenteric arteries. Am J Physiol Heart Circ Physiol. 2012 Feb 1. podcast: ajpheart.podbean.com/2012/03/.
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2010

Nausch, L.; Bonev, A.; Tallini, Y.; Michael, I.; Kotlikoff, M.; Nelson, M. (2010): Nerve-induced smooth muscle to endothelium signaling in small resistance arteries. FASEB J. 2010 24:598.7.

2008

Nausch, L.; Ledoux, J.; Bonev, A.; Nelson, M.; Dostmann, W. (2008): Differential patterning of cGMP in vascular smooth muscle cells revealed by single GFP-linked biosensors. PNAS 2008 105(1) 365–370.

Here, we report the design of unprecedented, non-FRET based cGMP-biosensors, named FlincGs, to assess the dynamics of nitric oxide (NO) and atrial natriuretic peptide (ANP) induced synthesis of intracellular cGMP, [cGMP](i). Regulatory fragments of PKG I alpha, PKG I beta, and an N-terminal deletion mutant of PKG I alpha were fused to circular permutated EGFP to generate alpha-, beta-, and delta-FlincG, with high dynamic ranges and apparent K(D,cGMP) values of 35 nM, 1.1 microM, and 170 nM, respectively. All indicators displayed significant selectivity for cGMP over cAMP, and 1.5- to 2.1-fold increases in fluorescence intensity at 510 nm when excited at 480 nm. Surprisingly, FlincGs displayed an additional excitation peak at 410 nm. delta-FlincG permitted ratiometric (480/410 nm) measurements, with a cGMP-specific 3.5-fold ratio change. In addition, delta-FlincG presented cGMP association and dissociation kinetics sufficiently fast to monitor rapid changes of [cGMP](i) in intact cells. In unpassaged, adenoviral transfected vascular smooth muscle (VSM) cells, delta-FlincG had an EC(50,cGMP) of 150 nM, and revealed transient global cGMP elevations to sustained physiological NO (EC(50,DEA/NO) = 4 nM), and the decay phase depended on the activity of PDE-5. In contrast, ANP elicited sustained submembrane elevations in [cGMP](i), which were converted to global cGMP elevations by inhibition of PDE-5 by sildenafil. These results indicate that FlincG is an innovative tool to elucidate the dynamics of a central biological signal, cGMP, and that NO and natriuretic peptides induce distinct cGMP patterning under the regulation of PDE-5, and therefore likely differentially engage cGMP targets.

2007

Nausch, L.; Dostmann, W. (2007): FlincGs: novel, non-FRET cGMP biosensors with nanomolar sensitivity for NO-induced signaling. BMC Pharmacology 2007, 7:S22.

Here, we report the design of unprecedented, non-FRET based cGMP-biosensors, named FlincGs, to assess the dynamics of nitric oxide (NO) and atrial natriuretic peptide (ANP) induced synthesis of intracellular cGMP, [cGMP]i. Regulatory fragments of PKG I α, PKG I β, and an N-terminal deletion mutant of PKG I α were fused to circular permutated EGFP to generate α-, β-, and δ-FlincG, with high dynamic ranges and apparent KD,cGMP values of 35 nM, 1.1 μM, and 170 nM, respectively. All indicators displayed significant selectivity for cGMP over cAMP, and 1.5- to 2.1-fold increases in fluorescence intensity at 510 nm when excited at 480 nm. Surprisingly, FlincGs displayed an additional excitation peak at 410 nm. δ-FlincG permitted ratiometric (480/410 nm) measurements, with a cGMP-specific 3.5-fold ratio change. In addition, δ-FlincG presented cGMP association and dissociation kinetics sufficiently fast to monitor rapid changes of [cGMP]i in intact cells. In unpassaged, adenoviral transfected vascular smooth muscle (VSM) cells, δ-FlincG had an EC50,cGMP of 150 nM, and revealed transient global cGMP elevations to sustained physiological NO (EC50,DEA/NO = 4 nM), and the decay phase depended on the activity of PDE-5. In contrast, ANP elicited sustained submembrane elevations in [cGMP]i, which were converted to global cGMP elevations by inhibition of PDE-5 by sildenafil. These results indicate that FlincG is an innovative tool to elucidate the dynamics of a central biological signal, cGMP, and that NO and natriuretic peptides induce distinct cGMP patterning under the regulation of PDE-5, and therefore likely differentially engage cGMP targets.

2002

Griesbeck, C.; Schutz, M.; Schodl, T.; Bathe, S.; Nausch, L.; Mederer, N.; Vielreicher, M.; Hauska, G. (2002): Mechanism of sulfide-quinone reductase investigated using site-directed mutagenesis and sulfur analysis. Biochemistry 2002 41(39):11552-65.

Biological sulfide oxidation is a reaction occurring in all three domains of life. One enzyme responsible for this reaction in many bacteria has been identified as sulfide:quinone oxidoreductase (SQR). The enzyme from Rhodobacter capsulatus is a peripherally membrane-bound flavoprotein with a molecular mass of approximately 48 kDa, presumably acting as a homodimer. In this work, SQR from Rb. capsulatus has been modified with an N-terminal His tag and heterologously expressed in and purified from Escherichia coli. Three cysteine residues have been shown to be essential for the reductive half-reaction by site-directed mutagenesis. The catalytic activity has been nearly completely abolished after mutation of each of the cysteines to serine. A decrease in fluorescence on reduction by sulfide as observed for the wild-type enzyme has not been observed for any of the mutated enzymes. Mutation of a conserved valine residue to aspartate within the third flavin-binding domain led to a drastically reduced substrate affinity, for both sulfide and quinone. Two conserved histidine residues have been mutated individually to alanine. Both of the resulting enzymes exhibited a shift in the pH dependence of the SQR reaction. Polysulfide has been identified as a primary reaction product using spectroscopic and chromatographic methods. On the basis of these data, reaction mechanisms for sulfide-dependent reduction and quinone-dependent oxidation of the enzyme and for the formation of polysulfide are proposed.


Beiträge zu wissenschaftlichen Konferenzen/Tagungen (peer-reviewed)

2018

Smetanska, I. (2018): Sustainable Production of Polyphenols and Anti-oxidants by Plant in vitro Cultures. . Eds. A Pavlov, T Bley, Bioprocessing of Plant in vitro Systems. Reference Series in Phytochemistry 2018, S.1-45.
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Sytar , O.; Beil , W.; Smetanska, I.; Brestic , M. (2018): Bioactive compounds and their biofunctional properties of different buckwheat germplasms for functional food. In: , , 1st Edition, ISBN: 978-0-12-811006-5. Buckwheat Germplasm in the World, edited by Drs. Zhou, Kreft, Tang and Suvorova 2018, S.191-204.


Zeitschriftenbeiträge

2018

Jaquemoth, M.; Hufnagel, R. (2018): Verbraucherpolitik. WISU - das Wirtschaftsstudium 47 (5), S.599-604.

2017

Voytsekhovskiy , V.; Smetanska, I. (2017): Packaging and storage of frozen berries. SWorld Journal , Yolnat PE, Minsk 2017 (13), S.229-234.

Jaquemoth, M.; Hufnagel, R. (2017): Mittagessen in der Kita – Qualität und Kosten. Ernährung im Fokus (05-06), S.144 ff.


Beiträge in Monografien, Sammelwerken und Schriftenreihen

2020

Nausch, L.; Uhlendorf, U.; Schadt, A.; Kommerell, D.; Wisura, L.; Schmidt, L. (2020): Nutri-Score: Verbrauchernutzen und Risiken am Beispiel der mediterranen Diät. Paracelsus Virtual Science Get Together der Paracelsus Medical University, Nürnberg/ Salzburg, Juni 2020.

2016

Gerschau, M. (2016): Marketing für Land- und Forstwirtschaft. Herausgeber: VELA; Schriftleitung: Eva-Maria Schmidtlein. Wirtschaftslehre und Betriebsmanagement. BLV Verlag, 14. Aufl., München 2016.

2013

Smetanska, I.; Barbosa-Cánovas , G.; Hunaefi, D. (2013): Non-thermal technologies in extending shelf-life of fresh-cut plant products: a review . Advances in Food Process Engineering Research and Applications. Food Engineering Series, S.375-415.
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2012

Janetzke, P. (2012): Der Beitrag der Künstlichen Intelligenz zur betrieblichen Prognose. Herausgeber: P. Mertens, S. Rässler; Prognoserechnung, 7.Aufl., Berlin u.a. 2012, S.341-382.


Bücher / Monografien

2018

Jaquemoth, M.; Hufnagel, R. (2018): Verbraucherpolitik: Ein Lehrbuch mit Beispielen und Kontrollfragen. Stuttgart 2018. ISBN 978-3-7910-3423-2 (280 Seiten).

Verbraucherpolitik ist durch die Globalisierung und Digitalisierung unserer Konsum- und Lebenswelt notwendiger denn je. Staatliche und nichtstaatliche Verbraucherarbeit suchen neue institutionelle Bahnen. In der Folge sind diverse Bachelor- und Masterstudienprogramme und Berufsfelder entstanden, die sich mit verbraucherpolitischen Fragestellungen befassen. Anhand von zahlreichen leicht verständlichen Fallstudien und Anwendungen gibt das Lehrbuch einen systematischen Überblick über Theorie und Praxis der Verbraucherpolitik. So können sich Studierende und Praktiker den gesamten Bereich der Verbraucherpolitik Stück für Stück anschaulich erarbeiten.

2014

Eckhardt, K. (2014): Hydrologische Modellierung - Ein Einstieg mithilfe von Excel. Springer-Verlag Berlin Heidelberg 2014.

Im vorliegenden Buch werden zwei Typen hydrologischer Modelle vorgestellt, das Niederschlag-Abfluss-Modell und das Grundwasserströmungsmodell. Mit Niederschlag-Abfluss-Modellen (N-A-Modellen) wird nachgebildet, wie im Einzugsgebiet eines Oberflächengewässers aus Niederschlag Abfluss wird. Modelle dieses Typs werden unter anderem eingesetzt, um Hoch- und Niedrigwasserabflüsse vorherzusagen oder um zu beurteilen, wie sich anthropogene Eingriffe wie Landnutzungsänderungen oder Speicherbewirtschaftung auf den Abfluss auswirken. Grundwasserströmungsmodelle dienen unter anderem dazu, die Verfügbarkeit von Grundwasser zur Gewinnung von Brauch- und Trinkwasser zu untersuchen, Grundwasserabsenkungen zu planen oder hydraulische Sicherungs- und Sanierungsmaßnahmen für Altlasten zu konzipieren.

2013

Eckhardt, K. (2013): Stochastik - Statistik und Wahrscheinlichkeitsrechnung in der Landwirtschaft. Ulmer-Verlag (utb GmbH), Stuttgart.

Messungen an sämtlichen Elementen einer Grundgesamtheit durchzuführen ist in der Regel jedoch nicht möglich oder nicht sinnvoll, zum Beispiel weil die Anzahl der Objekte zu groß ist und daher der Zeit- und/oder Kostenaufwand zu hoch wäre oder weil die Objekte bei der Untersuchung beschädigt oder zerstört werden (wie im Fall der Eier). Man muss sich dann (sollte sich dann) damit begnügen, aus der Grundgesamtheit eine Teilmenge von Objekten, eine Stichprobe, zu entnehmen und nur diese zu analysieren. In diesem Fall ist die Information über die Ausprägung des Merkmals in der Grundgesamtheit unvollständig. Das Ergebnis der Analyse weist daher eine gewisse Unsicherheit auf. Es gibt nicht den einen gesicherten Wert des Merkmals; man kann lediglich Aussagen über Intervalle machen, in welche die Merkmalswerte mit einer bestimmten Wahrscheinlichkeit fallen.

Statistik und Wahrscheinlichkeitsrechnung stehen daher in enger Verbindung. Die Wahrscheinlichkeitsrechnung dient außerdem beispielsweise dazu, Fragen der Genetik zu beantworten oder die Verlässlichkeit von Diagnose- und Prüfverfahren zu beschreiben.


Presseberichte und Medienbeiträge

2020

Newsmeldung HSWT, .; Noack, P. (2020): Digitalisierungsprojekt 'Diabek' kommuniziert mit den landwirtschaftlichen Zielgruppen über eine eigene Webseite sowie Social Media-Kanäle. Forschungs-News HSWT, 10.03.2020.
Volltext

Newsmeldung HSWT, .; Noack, P. (2020): Neues Forschungsprojekt der HSWT automatisiert die Verarbeitung drohnengestützt erhobener Bonituren in der Weizenzüchtung . Forschungs-News HSWT, 13.02.2020.
Volltext

2017

Newsmeldung HSWT, . (2017): Datenquellen für Landwirte miteinander verknüpfen: Verbundprojekt "AgriFusion" startet unter Beteiligung der HS Weihenstephan-Triesdorf. Forschungs-News der HSWT, 28.02.2017.
Volltext


Sonstige Veröffentlichungen

2015

Smetanska, I. (2015): Einfluss von Nährstoffversorgung und Stress auf die Biosynthese von phenolischen Substanzen (Fagopyrin, Rutin, Hypericin und Chlorogensäure) in Buchweizen.

2014

Shevchenko, Y.; Helfert, J.; Smetanska, I. (2014): Influence of stevia additives on antioxidant properties of different green teas. . Antioxidants, Agro FOOD Industry Hi Tech (24), S.22-26.

2008

Nausch, L. (2008): Novel Insights into PKG Activation and cGMP Signaling in Response to Nitric Oxide and Atrial Natriuretic Peptide in Vascular Smooth Muscle Cells. (2008). Graduate College Dissertations and Theses. 158.


Fakultät Landwirtschaft, Lebensmittel und Ernährung

Hochschule Weihenstephan-Triesdorf

Fakultät Landwirtschaft, Lebensmittel und Ernährung
Fakultät Landwirtschaft, Lebensmittel und Ernährung Markgrafenstraße 16
91746 Weidenbach

T +49 9826 654-131
lt [at]hswt.de

Betreuung der Publikationsseiten
Gerhard Radlmayr | Referent für Forschungskommunikation
T +49 8161 71-3350
kommunikation.zfw [at]hswt.de